Detection and enumeration of four foodborne pathogens in raw commingled silo milk in the United States

A nationwide survey was conducted to obtain qualitative and quantitative data on bacterial contamination of raw commingled silo milk intended for pasteurization. The levels of total aerobic bacteria, total coliforms, Enterobacteriaceae, Escherichia coli, and Staphylococcus aureus were determined using the TEMPO system. The prevalence rates and levels of presumptive Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella spp. were determined in 214 samples. Bacillus cereus was detected in 8.1% of samples, at 3.0 to 93 colony forming units (CFU) per ml. Escherichia coli O157:H7 was detected in 3.8% to 9.1% of samples, at 029-25.02.2013