In dairy goats, non-aureus Staphylococcus spp. (NAS) are the most common cause of subclinical mastitis. Many NAS species can cause intramammary infections (IMI) in goats, and pathogenicity seems to differ between NAS species. Data from cows also suggest that NAS species may occupy different ecological niches. However, more species-specific data are needed, which also requires more reliable species identification of NAS isolates, such as genotyping or other molecular-based methods.
The objectives of this PhD dissertation included the validation of MALDI-TOF mass spectrometry as a method for reliable species identification of staphylococci isolated from goats, the evaluation of the persistence of IMI during lactation and over the dry period using speciation and strain-typing, and the evaluation of the role of teat skin as a potential reservoir for IMI. A series of studies evaluating goats during lactation and over the dry period were conducted in three herds ranging in size from 42 to 1,400 lactating goats.
The typeability of MALDI-TOF for identifying staphylococcal species isolated from goats’ milk was 84%, with some v
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